Angela Johnese, director of the criminal justice office, and Heather Brantner, the mayor's Sexual Assault Response Team coordinator, left their city posts Friday. Officials declined to say under what terms they departed.
LeVar Michael, who led the city's anti-violence program Operation Ceasefire, left his position voluntarily a few weeks ago.
The City Council is expected to discuss the turnover and the mayor's strategy for addressing the surge in violence at a budget hearing Wednesday. This month has become the city's deadliest since 1999, with at least 35 people killed. More than 100 people have been killed in Baltimore this year.