Osemele was replaced in the lineup by backup center A.Q. Shipley.
"K.O.'s back is feeling better," Harbaugh said. "We'll just have to see throughout the course of the week how he responds."
The team is hopeful that starting wide receiver Marlon Brown could practice Wednesday after missing Sunday's game with a strained hamstring.
"It would be good to do that," Harbaugh said.
Defensive end Marcus Spears continues to get treatment on his knee after being sidelined Sunday. After the game, Spears told The Baltimore Sun that he doesn't expect this to be a long-term injury.
"It's nothing major or structural in there," Harbaugh said. "We'll just have to see how it responds. He'll probably be day-to-day."
"Arthur Brown had no issue with his pec strain, he appears to be 100 percent," Harbaugh said. "Webbie's hamstring got through it pretty well."
Wide receiver and kick returner Jacoby Jones will continue to practice this week.
Running back Ray Rice, who's no longer listed on the injury report with a strained left hip flexor, continues to get better, Harbaugh said.