Machado appeared to be in great pain as he held his right knee, and he had to be helped off the field by Orioles head athletic trainer Richie Bancells and manager Buck Showalter.
The Orioles announced later in the game that Machado had suffered a right knee sprain and would be re-evaluated Tuesday.
It was a scary moment that could have huge implications for Machado and the Orioles. He missed the first month of this season while recovering from surgery on his left knee, and he only recently has looked like the same player that was so productive in his first full major league season.
Machado tried to turn on a pitch from Yankees starter Chris Capuano and hit a ground ball to shortstop Derek Jeter, but he never got out the box. It appeared that Machado finished awkwardly on his left leg before his right knee gave way, but he was able to get up and walk gingerly off the field.
Regular first baseman Chris Davis, who was not in the starting lineup, replaced Machado at third base. The Orioles did not have another utility infielder available Monday because Ryan Flaherty already was filling in for the banged-up J.J. Hardy at shortstop.