The Wildcats, winners of seven of the last nine NCAA titles, will meet USC April 26 at 7 p.m.
Former Northwestern All-America Lindsey Munday is the USC coach.
It will be Northwestern’s final regular-season game of 2014. Tickets will be priced at $10 and go on sale Thursday.
While making the formal announcement Monday morning, Wildcats coach Kelly Amonte Hiller said the first opponent under consideration for a Wrigley Field game had been Notre Dame but the Irish move to the Atlantic Coast Conference made it impossible to work out a date.
In the past three seasons, the Northwestern women played in the first lacrosse games at Cowboys Stadium and the L.A. Coliseum and were part of the first women’s collegiate event -- a lacrosse doubleheader -- at the Patriots’ Gillette Stadium.